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KMID : 0880220120500040689
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Journal of Microbiology
2012 Volume.50 No. 4 p.689 ~ p.692
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The activity of phosphoinositide-specific phospholipase C is required for vegetative growth and cell wall regeneration in Coprinopsis cinerea
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Oh Young-Taek
Ahn Chun-Seob
Lee Kyung-Jin
Kim Jeong-Geun
Ro Hyeon-Su
Kim Jae-Won
Lee Chang-Won
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Abstract
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Three isotypes of phosphoinositide-specific phospholipase C designated CcPLC1, CcPLC2, and CcPLC3 were identified in Coprinopsis cinerea, through a search of the genome sequence database. The functional role of the PI-PLCs were studied by using U73122, which specifically inhibits the activity of PI-PLC. The specificity of the inhibitor effect was confirmed by using an inactive structural analog U73433. The inhibition of PI-PLCs activity resulted in severely retarded germination of basidiospores and oidia, reduced hyphal growth, knobbly hyphal tips with many irregular side branches, and aberrant (branch-like structure) clamp cells. Furthermore, U73122 definitely inhibited cell wall formation. Here we report that PI-PLCs play important roles in various aspects of C. cinerea biology.
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KEYWORD
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phosphoinositide-specific phospholipase C (PI-PLC), Coprinopsis cinerea, U73122
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